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KMID : 0620920220540122188
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Experimental & Molecular Medicine
2022 Volume.54 No. 12 p.2188 ~ p.2199
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SCON?a Short Conditional intrON for conditional knockout with one-step zygote injection
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Wu Szu Hsien Sam
Lee Hee-tak
Bakonyi Reka Szep
Colozza Gabriele
Boese Ayse
Gert Krista R.
Hallay Natalia
Lee Ji-Hyun
Kim Ji-hoon
Zhu Yi
Linssen Margot M.
Carotta Sandra Pilat
Hohenstein Peter
Theussl Hans-Christian
Pauli Andrea
Koo Bon-Kyoung
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Abstract
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The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189?bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
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